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目的:通过观察清化瘀毒方对酒精性大鼠肝纤维化转化生长因子-β1表达的影响,探讨清化瘀毒方抗酒精性肝纤维化的作用机制。方法:选择成年雄性Wistar大鼠,SPF级65只,随机分成正常对照组10只,造模组55只;按照复合因素制作酒精性大鼠肝纤维化模型,造模成功后,造模组采用随机分成3组,分别为复方鳖甲软肝片组10只,清化瘀毒方组10只和模型空白组10只;于第20周末次给药后禁食,全部处死取出肝脏进行HE染色及MASSON染色,免疫组化法检测肝脏组织TGF-β1表达。结果:实验性大鼠肝组织炎症活动度及肝纤维化程度:经清化瘀毒方治疗后,计分均明显降低,与模型空白组比较,差异有显著性意义(P<0.05)。免疫组化法检测酒精性大鼠肝脏TGF-β1的表达:与模型空白组比较,清化瘀毒方组明显降低,差异有显著性意义(P<0.05)。结论:清化瘀毒方能够显著减轻酒精性肝纤维化大鼠肝组织炎症活动度及纤维化程度,并能够降低酒精性肝纤维化大鼠肝组织中TGF-β1表达。
Objective: To observe the effect of Qinghuayuxijin on the expression of transforming growth factor-β1 in liver fibrosis induced by alcohol in rats and to explore the mechanism of anti-alcoholic liver fibrosis by Qinghuayu drug prescription. Methods: Adult male Wistar rats were randomly divided into normal control group (n = 10) and model group (n = 55). 65 adult SPF rats were randomly divided into three groups: model rats with alcoholic liver fibrosis were made according to the composite factors. Randomly divided into three groups, including Fufang Biejia Rugan Decoction 10, Qinghuayu Fu prescription 10 and model blank 10; at the end of the 20th week after the administration of fasting, all sacrificed to remove the liver HE staining And MASSON staining. The expression of TGF-β1 in liver tissue was detected by immunohistochemistry. Results: The degree of inflammatory activity and hepatic fibrosis in experimental rat liver tissue were significantly decreased after treatment with Qinghuayudu prescription. Compared with the model blank group, the difference was significant (P <0.05). The expression of TGF-β1 in alcoholic rat liver was detected by immunohistochemical method. Compared with the model blank group, Qinghuangyuxu prescription group was significantly lower, the difference was significant (P <0.05). Conclusion: Qinghua Yugan decoction can significantly reduce the degree of hepatic inflammation and fibrosis in alcoholic liver fibrosis rats and decrease the expression of TGF-β1 in the liver of alcoholic hepatic fibrosis rats.