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采用流动注射法研究了巯基化合物－铈（Ⅳ）－氢化可的松（ＨＣＲｓ）体系的化学发光行 为，对影响化学发光强度的诸因素进行试验和探讨，建立了流动注射化学发光法检测谷胱甘 肽（ＧＳＨ），半胱氨酸（Ｃｙｓ）等含巯基化合物的新方法。检测ＧＳＨ和Ｃｙｓ和线性范围分别为 ２．０×１０－６～１０．０×１０－５ｍｏｌ／Ｌ和１．０×１０－６ｍｏｌ／Ｌ～１０．０×１０－５ｍｏｌ／Ｌ；检测限分别为２．０ ×１０－７ｍｏｌ／Ｌ和１．４×１０－６ｍｏｌ／Ｌ（Ｓ／Ｎ＝３）；ＧＳＨ、Ｃｙｓ加入血清中进行回收测定的回收率 为９０％～９５％，相对标准偏差小于４％。
Flow injection was used to study the chemiluminescence behavior of thio compounds - cerium (Ⅳ) - hydrocortisone (HCRs) system. The effects of chemiluminescence intensity on the chemiluminescence intensity were studied and discussed. Flow injection chemiluminescence Glycopeptide (GSH), cysteine (Cys) and other mercapto compounds containing a new method. The linear range of detection of GSH and Cys were 2.0 × 10-6 ~ 10.0 × 10-5mol / L and 1.0 × 10-6mol / L ~ 10.0 × 10-5mol / L, respectively. The detection limits were 2.0 × 10-7mol / L and 1.4 × 10-6mol / L (S / N = 3). The recoveries of GSH and Cys added to serum were 90% ~ 95% .The relative standard deviations Less than 4%.