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目的观察不同剂量硝苯地平灌喂对大鼠肾草酸钙结石生成的影响。方法选用60只雄性SD大鼠,体重200~250g,随机分为6组,分别为空白对照组、单纯硝苯地平组、单纯诱石组、诱石+3、6、10mg·kg-1·d-1硝苯地平干预组,每组各10只。应用乙二醇诱导大鼠产生肾草酸钙结石,4周后观察大鼠肾小管结晶沉积情况、肾组织自由基水平、细胞凋亡情况和大鼠血和尿多项生化指标的变化。结果与单纯诱石组相比,各硝苯地平干预组的肾小管草酸钙结晶评分分别降低37.0%、55.6%、66.7%(P均<0.05),肾小管上皮细胞凋亡数分别减少30.2%、44.6%、48.7%(P均<0.05),两者有显著相关性(r=0.8251);肾组织中MDA含量也分别减少14.5%、20.4%、21.8%,而SOD活性增加3.5%、8.7%、12.6%(P均<0.05);量效关系呈正相关。结论硝苯地平通过减少高尿草酸所致的肾小管上皮细胞凋亡,能有效抑制饮用诱石剂大鼠的肾小管结石生成。
Objective To observe the effects of different doses of nifedipine on the formation of renal calcium oxalate stones in rats. Methods Sixty male Sprague-Dawley rats weighing 200-250 g were randomly divided into 6 groups: blank control group, simple nifedipine group, simple lure group, lure + 3,6 and 10 mg · kg -1 d-1 nifedipine intervention group, each group of 10. Application of ethylene glycol induced rat renal calcium oxalate stones, 4 weeks after the observation of rat renal tubular deposition, free radical levels of renal tissue, apoptosis and blood and urine of multiple biochemical indicators. Results The renal tubular calcium oxalate score decreased by 37.0%, 55.6%, 66.7% (P <0.05), and the number of renal tubular epithelial cell apoptosis decreased by 30.2% , 44.6% and 48.7%, respectively (all P <0.05), and there was a significant correlation between them (r = 0.8251). The content of MDA in renal tissue decreased by 14.5%, 20.4% and 21.8% %, 12.6% respectively (all P <0.05). There was a positive correlation between the dose-effect and the control. Conclusion Nifedipine can inhibit renal tubular epithelial cell apoptosis induced by high urinary oxalic acid and can inhibit the formation of renal tubules in drinking-inducing rats.