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目的比较不同标本采集条件对EV71病毒核酸检测结果的影响,加强手足口病标本采集工作。方法对菏泽市11家手足口病治疗定点医院采集的标本做RT-PCR检测;依据不同条件对EV71阳性率做统计学分析。结果 EV71阳性率在不同病例年龄关系、病例所在地、是否重症3种情况下,差异无统计学意义;而在不同病例标本采集时间、采集地、病例性别差异三种条件下,EV71阳性率出现了明显的统计学差异。结论 EV71阳性率与病例标本的采集时间、采集地以及病例性别关系三者之间有明显的关系。
Objective To compare the effect of different specimen collection conditions on the detection results of EV71 virus nucleic acid and to enhance the collection of hand, foot and mouth disease specimens. Methods The specimens collected from 11 hand-foot-mouth disease hospitals in Heze City were detected by RT-PCR. The positive rates of EV71 were analyzed according to different conditions. Results The positive rate of EV71 was not significantly different among the three groups in terms of age, case location, and severity of disease. However, the positive rate of EV71 was found in three different conditions: collection time, collection site and case gender Significant statistical differences. Conclusion There is a clear relationship between the positive rate of EV71 and the collection time of the specimen, the place of collection and the gender of the cases.