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目的 :探讨抑癌基因 p5 3外显子 7- 8突变在宫颈癌发生中的作用和意义。方法 :聚合酶链反应(PCR)、单链构象多态性 (SSCP)分析。结果 :15 0例不同病变宫颈组织中均未见 p5 3外显子 7- 8缺失 ;可见 p5 3外显子 7- 8突变在宫颈癌、宫颈不典型增生、宫颈息肉组织中分别为 14.2 9% (7/ 49)、16 % (8/ 5 0 )、3.85 % (1/ 2 6 ) ;宫颈慢性炎症组织中未见突变。p5 3外显子 7- 8突变在宫颈癌、宫颈不典型增生和宫颈息肉之间无显著差异 (χ2 =0 .36 1,P>0 .5 ) ;且与宫颈癌的分化程度、浸润深度及宫颈不典型增生的病理分级无显著相关性 (r<0 .997,P>0 .0 5 )。结论 :在宫颈癌发生中 p5 3外显子 7- 8突变是一个重要且早期的致癌因素
Objective: To investigate the role and significance of mutation of p5-3 gene exon 7-8 in cervical carcinogenesis. Methods: Polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP) analysis. RESULTS: No deletion of p5 3 exon 7 -8 was found in 150 cases of cervical lesion. The mutation of p5 3 exon 7-8 in cervical cancer, cervical dysplasia and cervical polyps was 14.29 (7/49), 16% (8/50), 3.85% (1/2 6) respectively. There was no mutation in the chronic inflammation of cervix. The mutation of p5 3 exon 7-8 had no significant difference between cervical cancer, cervical dysplasia and cervical polyps (χ2 = 0.361, P> 0.05), and it also correlated with the degree of differentiation, depth of invasion And cervical dysplasia pathological grade no significant correlation (r <0. 997, P> 0.05). Conclusions: The mutation of p5 3 exon 7 -8 in cervical carcinogenesis is an important and early carcinogenic factor