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据文献报道,mCALPAIN(CALCIUM ACTIVATED NEUTRAL PROTEINASE Ⅱ)在轴突及髓鞘的演变中起关键作用,并与细胞骨骼蛋白质的更新和退变有关.为研究这种蛋白酶在脑缺血时神经元受损中所起的作用,用免疫细胞化学方法检查了这种酶在脑缺血后的表达.取10只SD大白鼠,按CHEN等氏改良法造成大脑单侧局部缺血动物模型(永久性阻断左侧大脑中动脉的主支的同时,暂时阻断双侧颈总动脉).动物分二批,存活中各为6/小时及24小时,行心内灌注固定.取大脑运动区按常规作恒冷箱冰冻切片,片厚40μm,每6片取1片,贴片按ABC法行免疫细胞化学法检验.另一套片用NISSL染色.在大脑运动区尼氏染色及mCALPAIN样免疫反应性均在局部缺血区与非缺血区出现明显分界线.6小时组在缺血区Ⅱ～Ⅴ层神经元的尼氏染色减弱,而mCALPAIN样免疫反应性明显增强,并随存活时间增加而更甚,24小时组的尼点染色更弱,而mCALPAIN样免疫反应性更强.结果提示,这种蛋白酶可能与迟发性神经元损害有关.与我们以前研究实验性脊髓损伤动物的结果相似.
It has been reported in the literature that mCALPAIN (CALCIUM ACTIVATED NEUTRAL PROTEINASE II) plays a key role in the evolution of axons and myelin and is related to the renewal and degeneration of cellular skeletal proteins.In order to investigate the role of this protease in neuronal activation during cerebral ischemia Damage in the role played by the immunocytochemical method to check the expression of this enzyme in the brain after ischemia. Take 10 SD rats, according to CHEN et al improved method of causing unilateral cerebral ischemia in animal models (permanent Blocking the main branch of the left middle cerebral artery at the same time, temporarily blocking the bilateral common carotid artery.) Animals were divided into two batches, each of survival 6 / hour and 24 hours, perfusion fixed intracardiac area Conventional cryostat freeze-thaw section, thickness 40μm, take a slice of every 6, the patch according to ABC method of immunocytochemistry test.Another set of slices with NISSL staining in the brain area of motion Nissl staining and mCALPAIN-like immunity Reactivity in the ischemic and non-ischemic areas showed a clear dividing line .6 hours group ischemic area II ~ V neurons Nissl staining decreased, and mCALPAIN-like immunoreactivity was significantly enhanced, and with the survival time Increased even more, 24 hours group Nissl staining was weaker, and mC ALPAIN-like immunoreactivity was stronger, suggesting that this protease may be associated with delayed neuronal damage similar to what we did with previous studies of experimental SCI animals.