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目的：在多因素串联色谱响应函数（ＨＣＲＦ） 基础上提出了新的优化指标ｇＨＣＲＦ，对新化合物氯苄律定有关物质的ＨＰＬＣ 分离进行优化。方法：采用球面对称设计，对影响色谱分离的两个主要因素（ｘ１ ：甲醇比例；ｘ２ ：离子对试剂浓度） 进行优化，并对试验结果进行关系式模拟。结果：以ｇＨＣＲＦ 建立的关系式预测的理论最优解为ｘ１ ＝７６ ％ ，ｘ２ ＝ ０－０１ ｍｏｌ·Ｌ－ １ ，与实验最优解ｘ１ ＝７５％ ，ｘ２ ＝ ０－０１ ｍｏｌ·Ｌ－１ 十分接近，预测能力可信。结论：氯苄律定有关物质的分离证实ｇＨＣＲＦ适用于复杂混合样品的分离优化
OBJECTIVE: To optimize the HPLC separation of chlorobenzidine-related substances of new compound gHCRF based on the multi-factor tandem chromatographic response function (HCRF). Methods: The spherical symmetry design was used to optimize the two main factors affecting the chromatographic separation (x1: methanol ratio; x2: ion-pair reagent concentration), and the experimental results were modeled. Results: The theoretical optimal solution predicted by the relation of gHCRF was x1 = 76%, x2 = 0-01 mol·L-1, which was in agreement with the experimental optimal solution x1 = 75%, x2 = 0-01 mol·L- 1 is very close, predictable ability credible. CONCLUSIONS: The separation of the relevant substances from the chlorobenzylidine protocol confirms that gHCRF is suitable for the separation and optimization of complex mixtures