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本文应用非损伤微测技术(non-invasive micro-test technique,NMT)研究了H2S对盐胁迫下耐盐的胡杨和不耐盐的群众杨根部K+、Na+和H+流速的影响。结果表明,两种杨树根系在瞬时(100mmol·L-1NaCl)和短期(24h,50mmol·L-1NaCl)盐胁迫下呈现出K+外流,这种盐诱导的根部K+外流能被50mol·L-1NaHS抑制。此外,H2S能增加短期盐胁迫下Na+的外排,而Na+的外流被Na+/H+逆向转运体抑制剂amiloride或者质膜H+泵抑制剂sodium orthovanadate有效抑制。盐胁迫下,H2S对不耐盐的群众杨根系K+外流的抑制作用和对Na+/H+逆向转运的促进作用高于耐盐的胡杨。据此推测,H2S是通过上调杨树根系胞质膜Na+/H+逆向转运体系(H+泵和Na+/H+逆向转运体),促进Na+和H+逆向跨膜转运,并且,H+泵通过抑制去极化激活的离子通道来限制盐诱导的K+外流。
In this paper, we studied the effects of H2S on K +, Na + and H + flux of salt-tolerant Populus euphratica and salt-tolerant Populus tomentosa populations under non-invasive micro-test technique (NMT). The results showed that K + efflux appeared under both salt stress (100 mmol·L-1 NaCl) and short-term (24h, 50 mmol·L-1 NaCl) salt stress in both poplar roots. The salt-induced root K + efflux was inhibited by 50 mol·L- 1NaHS inhibition. In addition, H2S increased the efflux of Na + under short-term salt stress, while outflow of Na + was inhibited by Na + / H + antiporter inhibitor amiloride or plasma membrane H + pump inhibitor sodium orthovanadate. Under salt stress, the inhibitory effect of H2S on K + efflux of poplar roots and the promotion of Na + / H + reverse transport were higher than those of salt-tolerant Populus euphratica. It is speculated that H2S promotes the reverse transmembrane transport of Na + and H + by up-regulation of the Na + / H + antiporter (H + pump and Na + / H + antiporter) in poplar roots and inhibits the depolarization Activated ion channels to limit salt-induced K + efflux.