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目的:研究和建立大承气汤汤剂HPLC指纹图谱,为该方的物质基础和质量控制提供了科学依据。方法:采用ZORBAX SB-C18色谱柱(4.6mm×250mm,5μm),甲醇-0.1%磷酸水溶液为流动相,梯度洗脱,流速1mL/min,检测波长220nm,柱温25℃。以柚皮苷为参照物,在相同的色谱条件下测定10批大承气汤汤剂。结果:建立了大承气汤汤剂的HPLC指纹图谱,标定了29个共有指纹峰,通过与对照品的保留时间及紫外光谱比较,指认了芸香柚皮苷、新橙皮苷、柚皮苷、橙皮苷、大黄酸、大黄酚、和厚朴酚、厚朴酚的出峰位置,并进行峰位归属,10批样品的相似度在0.981～0.997之间,该HPLC方法的精密度、稳定性、重现性良好。结论:建立的方法准确、可靠,对大承气汤物质基础的研究提供了一定参考。
Objective: To study and establish the HPLC fingerprints of Dachengqi decoction, which provides a scientific basis for the material basis and quality control of this prescription. METHODS: ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of methanol and 0.1% phosphoric acid. The flow rate was 1 mL / min and the detection wavelength was set at 220 nm. Using naringin as a reference, 10 batches of Dachengqi Decoction were tested under the same chromatographic conditions. Results: The HPLC fingerprint of Dachengqi decoction was established and 29 common fingerprint peaks were calibrated. Compared with the retention time and ultraviolet spectrum of reference substance, Rutin, naringin, neohesperidin and naringin were identified , Hesperidin, rhein, chrysophanol, honokiol and honokiol peak position, and the peak position attribution, the similarity of 10 batches of samples between 0.981 ~ 0.997, the HPLC method of precision, Stability, reproducibility is good. Conclusion: The established method is accurate and reliable, and provides a reference for the research on the material basis of Dachengqi Decoction.