目的:探讨水通道蛋白8(AQP8)在人脑胶质瘤中的表达及其与预后的关系。方法:选取河南省人民医院2017年7月至2019年7月35例人脑胶质瘤组织，通过实时荧光定量聚合酶链反应(PCR)技术检测AQP8的表达，再通过短发卡RNA(shRNA)技术下调AQP8基因，通过细胞增殖测定和Transwell侵袭/转移实验研究其对人脑胶质瘤U373和T98G细胞增殖和侵袭/迁移的影响。采用n t检验或方差分析进行组间差异比较。n 结果:AQP8的表达水平在胶质瘤中高于正常脑组织(1.52±0.36，n t＝7.969，n P<0.01)，差异有统计学意义；下调AQP8后，在U373和T98G细胞的72 h相对增殖率显著低于对照组(0.68±0.27，0.64±0.30，n t＝11.043、1.110，n P<0.01)，差异有统计学意义，同时在这两种细胞中细胞周期蛋白CCNB1(0.88±0.17，0.64±0.15，n t＝6.263，n P<0.01)，CCNB2(0.18±0.05，0.11±0.06，n t＝5.302，n P<0.01)，CDK1(0.68±0.14，0.41±0.12，n t＝8.663，n P<0.01)，KIF4A(0.47±0.19，0.19±0.06，n t＝8.314，n P<0.01)和FEN1(0.31±0.06，0.26±0.05，n t＝3.787，n P<0.01)的表达也明显降低，差异有统计学意义。此外，干预组U373和T98G细胞的侵袭/迁移能力显著下降。n 结论:下调AQP8可抑制胶质瘤细胞的增殖并降低其侵袭和迁移能力。“,”Objective:To investigate the expression of aquaporin 8 (AQP8) in human glioma and its relationship with prognosis.Methods:The expression of AQP8 was detected by real-time quantitative polymerase chain reaction (PCR) in 35 cases of human glioma tissues, and then AQP8 gene was down-regulated by short hairpin RNA (shRNA) technology. The effects of AQP8 on the proliferation and invasion/migration of u373 and T98G cells were studied by cell proliferation assay and Transwell invasion/metastasis experiment. n T test or analysis of variance was used to compare the differences between groups.n Results:AQP8 expression level was up-regulated in glioma (1.52±0.36) as compared with normal brain tissue (n t=7.969, n P<0.01). After down-regulating AQP8, the relative proliferation rate of u373 and T98G cells was decreased at 72 h as compared with normal brain tissue (0.68±0.27, 0.64±0.30,n t=11.043, 1.110, n P<0.01). The expression of cyclins in u373 and T98G cells CCNB1 (relative gray value, 0.88±0.17, 0.64±0.15,n t=6.263, n P<0.01), CCNB2 (relative gray value, 0.18±0.05, 0.11±0.06,n t=5.302, n P<0.01), cyclin dependent kinase 1 (CDK1, relative gray value, 0.68±0.14, 0.41±0.12,n t=8.663, n P<0.01), KIF4A (relative gray value, 0.47±0.19, 0.19±0.06,n t=8.314, n P<0.01) and FEN1 (relative gray value, 0.31±0.06, 0.26±0.05,n t=3.787, n P<0.01) was significantly decreased. In addition, the invasion/migration ability of u373 and T98G cells was significantly decreased in the intervention group.n Conclusion:Down-regulation of AQP8 can inhibit the proliferation, invasion and migration of glioma cells.