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目的研究脆性组氨酸三联体基因(FHIT)在由正常宫颈上皮过度至子宫颈癌过程中的缺失情况,探讨FHIT基因缺失在子宫颈癌发生发展中的作用及其临床意义。方法应用RT-PCR法对36例正常子宫颈组织、104例宫颈上皮内瘤样病变(CIN)、138例浸润性宫颈癌中FHIT杂合性缺失情况进行分析,并与组织学类型、组织学分级及临床分期等进行相关分析。结果 FHIT基因在正常子宫颈组织、宫颈CIN、浸润性宫颈癌中杂合性缺失率分别为0%、30.8%、66.7%。正常组、CIN组、CC组三组比较差异有统计学意义(P<0.05)。结论 FHIT基因缺失贯穿在宫颈癌发生发展的整个过程中,起始于癌前病变,并且随着细胞恶性程度的增加缺失率逐步增高。FHIT基因检测可用于宫颈癌的筛查及早期诊断。
Objective To investigate the role of FHIT in the deletion of normal cervical epithelium to cervical cancer and to explore the role of FHIT deletion in the development of cervical cancer and its clinical significance. Methods The loss of heterozygosity of FHIT in 36 cases of normal cervical tissue, 104 cases of cervical intraepithelial neoplasia (CIN) and 138 cases of invasive cervical carcinoma were analyzed by RT-PCR. The differences of histological type, Grade and clinical stage related analysis. Results The loss rate of heterozygosity of FHIT gene in normal cervical tissue, cervical CIN and invasive cervical cancer were 0%, 30.8% and 66.7% respectively. The difference between the normal group, CIN group and CC group was statistically significant (P <0.05). Conclusion FHIT gene deletion throughout the development of cervical cancer throughout the process, starting from precancerous lesions, and as the degree of cell malignancy increased the loss rate gradually increased. FHIT gene test can be used for screening and early diagnosis of cervical cancer.