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已知核基质的功能与细胞的分化、增殖等生命活动过程有关,且与细胞的癌变和凋亡有关[1],我们比较了肿瘤坏死因子诱导HL60细胞凋亡前及凋亡时核基质的改变,并分析了核基质蛋白在HL60细胞凋亡过程的可能作用及其意义。材料与方法1.HL60细胞的培养的准备:HL60细胞为本实验室
The function of the nuclear matrix is known to be related to cell life cycle such as differentiation and proliferation, and is related to the canceration and apoptosis of cells [1]. We compared the TNF-induced apoptosis of HL60 cells before and during apoptosis. Changes, and analyzed the possible role of nuclear matrix protein in the apoptosis of HL60 cells and its significance. Materials and Methods 1. Preparation of HL60 Cell Culture: HL60 Cell-based Laboratory