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目的 观察中国新生儿脐带血和成人外周血淋巴细胞和单核细胞分泌巨噬细胞炎症蛋白 1α(MIP- 1α)、MIP - 1β和受激活调节的正常T淋巴细胞表达和分泌因子 (RANTES)的能力 ,探讨影响人免疫缺陷病毒(HIV)母婴传播的可能因素。方法 Ficol密度梯度离心、贴附法分离淋巴细胞和单核细胞 ,并以免疫荧光CD3、CD14单克隆抗体鉴定。分别在PMA或LPS刺激下培养 ,ELISA法检测培养上清中三种CC趋化因子浓度。结果 17例新生儿脐带血淋巴细胞和单核细胞分泌MIP - 1α、MIP - 1β、RANTES的浓度分别为 392 0± 730、4 910± 5 90、1470± 4 10、32 4 0± 980、196 0± 130 0、2 4 0± 12 0pg/ml,2 0例成人外周血淋巴细胞和单核细胞分泌MIP - 1α、MIP - 1β、RANTES的浓度分别为 6 5 6 0± 84 0、5 810± 115 0、2 2 5 0± 5 70、30 10± 135 0、2 2 80± 870、6 90± 4 30pg/ml。脐带血淋巴细胞和单核细胞上述分泌能力低于成人血。结论 中国新生儿脐带血淋巴细胞和单核细胞分泌RANTES的能力低下 ,可能使中国新生儿比成人对HIV - 1更易感且影响发病过程。应在新生儿对HIV - 1感染的自动免疫和被动免疫处理时采取相应对策
OBJECTIVE: To observe the secretion of macrophage inflammatory protein 1α (MIP-1α), MIP-1β and RANTES activated by normal neonatal umbilical cord blood and adult peripheral blood lymphocytes and monocytes Ability to explore possible factors that affect the transmission of human immunodeficiency virus (HIV) from mother to child. Methods Ficol density gradient centrifugation and attachment method were used to isolate lymphocytes and mononuclear cells and identified by immunofluorescence staining with CD3 and CD14 monoclonal antibodies. The cells were cultured in the presence of PMA or LPS, and the concentrations of three CC chemokines in the culture supernatants were detected by ELISA. Results The concentrations of MIP - 1α, MIP - 1β and RANTES secreted by umbilical cord blood lymphocytes and monocytes from 17 neonates were 392 0 ± 730,4 910 ± 5 90,1470 ± 4 10,32 4 0 ± 980,196 The concentrations of MIP - 1α, MIP - 1β and RANTES in 20 adult peripheral blood mononuclear cells and monocytes were 0 ± 130 0,2 4 0 ± 12 0 pg / ml, respectively. The concentrations of RANTES were 6 56 0 ± 84 0,5 810 ± 115 0,2 2 5 0 ± 5 70,30 10 ± 135 0,2 2 80 ± 870,6 90 ± 4 30 pg / ml. Umbilical cord blood lymphocytes and monocytes below the secretion capacity of adult blood. Conclusion The low ability of cord blood lymphocytes and monocytes secreting RANTES in newborn infants in China may make Chinese newborns more susceptible to HIV - 1 and affect the pathogenesis than adults. Corresponding countermeasures should be taken in neonates with autoimmune and passive immunization of HIV - 1 infection