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目的了解莱州市蜱的种类以及蜱携带发热伴血小板减少综合征新布尼亚病毒的感染状况,为证实当地发热伴血小板减少综合征的传播媒介提供科学依据。方法在莱州市有发热伴血小板减少综合征病例发生的自然村,收集动物体表的寄生蜱和采用布旗法收集野外的游离蜱,开展蜱的分类鉴定,并采用实时荧光定量PCR进行新布尼亚病毒的病原学检测。结果 2011年在莱州市共采集到2 593只蜱。按蜱的种类分类,莱州地区共采集到长角血蜱2 573只和血红扇头蜱6只,最低感染率分别为4.20%和16.67%;采集到铃头血蜱9只和中华革蜱5只,未检测到新布尼亚病毒核酸阳性。按照蜱的不同发育时期分类,共采集到成蜱1 449只、若蜱995只、幼蜱149只,成蜱、若蜱和幼蜱的最低感染率分别为4.07%、4.52%和2.68%;实验室培养期间收获22份卵,其中2份卵新布尼亚病毒核酸检测阳性,最低感染率为9.09%;按照动物来源分类,共采集羊蜱1 435只、狗蜱74只、牛蜱128只、鸡蜱61只、刺猬蜱24只,除鸡蜱、刺猬蜱未检测到新布尼亚病毒核酸外,羊蜱、狗蜱和牛蜱中SFTSV最低感染率分别为5.37%、12.16%和1.56%;野外采集蜱871只,最低感染率为2.18%。结论不同发育时期的蜱均有不同程度的新布尼亚病毒感染;长角血蜱、血红扇头蜱可能为新布尼亚病毒的传播媒介,且新布尼亚病毒具有垂直传播的可能。
Objective To understand the species of tick and the infection status of tick-borne fever with nebulized Bunyavirus in Laizhou and provide a scientific basis for confirming the local fever and thrombocytopenic syndrome. Methods In natural villages with fever and thrombocytopenia syndrome in Laizhou City, parasitic ticks on the surface of animals were collected, and wild ticks were collected in the wild by using the cloth flag method. The ticks were classified and identified by real - time PCR. Sub-virus etiology testing. Results A total of 2 593 ticks were collected in Laizhou City in 2011. According to the classification of ticks, a total of 2 573 H. longicornis and 6 R. anguillas were collected in Laizhou area, with the lowest infection rates of 4.20% and 16.67%, respectively. Nine ticks of ticks and 5 Only, no new Bunyavirus nucleic acid was detected. A total of 1 449 adult ticks, 995 ticks and 149 ticks were collected according to their different developmental stages. The lowest infection rates of ticks, ticks and ticks were 4.07%, 4.52% and 2.68%, respectively. Twenty-two eggs were harvested during laboratory culture, of which two were positive for neubrian virus with the lowest infection rate of 9.09%. According to animal source classification, a total of 1 435 sheeps, 74 dogs and 128 The lowest SFTSV infection rates were 5.37%, 12.16% and 1.56 in goat, tick and ticks, respectively, except for 61 chickens and 24 hedgehogs. %; 871 ticks were collected in the field, the lowest infection rate was 2.18%. Conclusion All the ticks at different developmental stages have different levels of infection with New Bunyan virus. The H. longicornis and R. sanguineus may be the vectors of new Bunyan virus and the new Bunyan virus may spread vertically.