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ELISA测定HBsAg具有灵敏度高,特异性强,准确性好等优点,被各级大中小医院检验室广泛采用。ELISA二步技术,试验时间冗长,给操作人员带来一定的不便。ELISA一步法将待测样品与酶标抗体一次加入,时间短,操作简单,但是,一步法在检测高滴度HBsAg时易出现前带现象,得假性结果。我科对门诊及住院病人用血清标本作ELISA一步法及二步法平行对照试验,并与反向血凝法(RPHA)进行比较分析。对ELISA一步法阴性结果标本,可用RPHA再次检测HBsAg,并排除假阴性结果,而保证了检测结果的准确性。
ELISA assay HBsAg has the advantages of high sensitivity, specificity, accuracy and so on. It has been widely adopted by laboratories of large, medium and small hospitals at all levels. ELISA two-step technology, long test time, to the operator to bring some inconvenience. ELISA one-step method to be tested samples and enzyme-labeled antibody once added, time is short, easy to operate, however, one-step method in the detection of high titer HBsAg prone to the former with the phenomenon of false results. Our department of outpatients and inpatients with serum samples for ELISA one-step and two-step parallel control trial and compared with reverse hemagglutination (RPHA) were analyzed. Negative results of ELISA one-step results of specimens, RPHA can be used again to detect HBsAg, and exclude false-negative results, and to ensure the accuracy of the test results.