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将血吸虫肠相关阴极抗原的单克隆抗体3D8A联结过氧化物酶后用于直接法Dot-ELISA检测急性、慢性与晚期血吸虫病人血清中循环抗原,阳性率分别为90.6、83.2及30.7％。正常人血清及非寄生虫感染病人血清均未见阳性反应。EPG>100组血清阳性率及抗原水平高于EPG<100。慢性血吸虫病人经吡喹酮治疗后一年,粪检阴性者大部分血清抗原转为阴性;粪检阳性者血清检查仍为阳性。结果表明,肠相关阴极抗原单克隆抗体3D8A能用于Dot-ELISA检测各期病人血清中循环抗原,并能反映病人感染度与药物治疗效果。
The monoclonal antibody 3D8A of Schistosoma japonicum associated antigen was used to detect circulating antigens in the serum of patients with acute, chronic and advanced schistosomiasis by direct ELISA with Dot-ELISA. The positive rates were 90.6, 83.2 and 30.7% respectively. Normal serum and non-parasitic infections in patients with serum no positive reaction. Serum positive rate and antigen level in EPG> 100 group were higher than EPG <100. One year after chronic schistosomiasis treatment with praziquantel, most of the serum antigens in negative stool tests turned negative; the positive stool tests were still positive in stool tests. The results showed that 3D8A, a monoclonal antibody against gut-associated antigen, could be used to detect circulating antigens in serum of patients with different stages by Dot-ELISA and could reflect the patient’s infection and drug treatment.