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为了探讨三氧化二镍 (Ni2 O3)诱导人肺成纤维 (HL F)细胞恶性转化作用以及β-胡萝卜素和维生素C的保护作用 ,用不同浓度的 Ni2 O3在体外多次处理 HL F细胞 ,利用刀豆凝集素 A(Con A)凝集实验和软琼脂集落形成实验进行转化细胞的恶性鉴定 ;同时添加β-胡萝卜素 (5 .4mg/ L )和维生素 C的食物 (1.8mg/ L ) ,观察对 Ni2 O3转化作用的影响。结果表明 Ni2 O3(0 .5～ 2 .0 mg/ L)可诱导 HL F恶性转化 ,转化细胞生长速度加快 ,排列紊乱 ,失去接触抑制 ,呈交叉重叠生长 ,转化率呈剂量 -效应关系。转化细胞可被低浓度 Con A凝集 ,并在软琼脂内生长。添加 β-胡萝卜素和维生素 C的处理组比单用 Ni2 O3 组的转化率显著下降 (P<0 .0 5 ) ,处理后细胞 Con A凝集反应阴性 ,不能在软琼脂内生长。结论认为 Ni2 O3有较强的诱导 HL F细胞恶性转化的能力 ,具有致癌性。β-胡萝卜素和维生素 C对于镍诱导 HL F恶性转化具有保护作用 ,提示职业接触镍的人群有必要在膳食中增加富含β-胡萝卜素和维生素 C,以提高机体的抗氧化能力 ,从而提高机体的抗肿瘤能力
To investigate the malignant transformation of human lung fibroblasts (HLF) induced by nickel oxide (Ni2 O3) and the protective effect of β-carotene and vitamin C, HL F cells were treated with Ni2 O3 in different concentrations for several times, Malignant identification of the transformed cells was carried out by Con A agglutination test and soft agar colony formation assay. At the same time, β-carotene (5.4 mg / L) and vitamin C (1.8 mg / L) The effect on the conversion of Ni2 O3 was observed. The results showed that Ni2 O3 (0 .5 ~ 2.0 mg / L) could induce the malignant transformation of HLF. The growth of transformed cells was accelerated, disordered and lost contact inhibition, with overlapping and overlapping growth. The conversion rate showed a dose-response relationship. Transformed cells can be condensed with low concentrations of Con A and grow in soft agar. The conversion of β-carotene and vitamin C-treated group was significantly lower than that of the single Ni2 O3-treated group (P <0.05). After treatment, the cells were negative for Con A agglutination and could not grow in soft agar. Conclusion Ni2 O3 has strong ability to induce malignant transformation of HL F cells and is carcinogenic. Beta-carotene and vitamin C have a protective effect on nickel-induced malignant transformation of HLF, suggesting that it is necessary for occupational exposure to nickel to increase beta-carotene and vitamin C in the diet to enhance the body’s antioxidant capacity and thereby improve Anti-tumor ability of the body