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建立反转录聚合酶链反应(RT-PCR)检测庚型肝炎病毒感染的方法。该法特异性强,检测下限(1.9×103)与感染剂量相当,简便实用。对我国各类人群的HGV感染情况进行研究,结果表明,慢性丙型肝炎患者、抗-HCV阳性职业献血员和已知肝炎病毒标志物均阴性者感染率较高,分别为60%、40%和21.7%。对其中一株(P11)阳性扩增产物所做部分核酸序列分析,与国外两株相应区段的同源性高达96.4%和95.3%。
To establish a reverse transcription polymerase chain reaction (RT-PCR) detection of Hepatitis G virus infection method. The specificity of this method, the detection limit (1.9 × 103) and the dose of infection is quite simple and practical. The study of HGV infection in all kinds of people in China showed that the infection rate of patients with anti-HCV positive blood donors and those with negative hepatitis B virus markers was higher in patients with chronic hepatitis C, 60% and 40% And 21.7%. The partial nucleic acid sequence analysis of one of the positive amplification products (P11) showed 96.4% and 95.3% homology with the corresponding two foreign isolates.