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目的:探讨影响乌拉尔甘草愈伤组织诱导的因素,建立乌拉尔甘草愈伤组织培养方法。方法:采用组织培养法,比较不同外植体、光照、植物生长物质种类及其配比对乌拉尔甘草愈伤组织诱导和生长的影响。结果:不同外植体中,下胚轴愈伤组织诱导率最高(平均94%),且发生最早,愈伤化程度高,筛选出下胚轴是诱导愈伤组织最佳外植体,在MS+6-BA(1.0~2.0)mg/L+NAA(0.5~1.0)mg/L培养基上,愈伤组织可分化产生不定芽;2,4-D诱导产生非胚性愈伤组织,一定浓度的6-BA与NAA配合,诱导产生胚性愈伤组织;光照影响愈伤组织的诱导及生长。结论:不同外植体、激素含量及光照是影响乌拉尔甘草愈伤组织诱导和生长的主要因子。
Objective: To explore the factors that affect the callus induction of Ural licorice and establish the callus culture method of Ural licorice. Methods: Tissue culture method was used to compare the effects of different explants, light and plant growth substances on the callus induction and growth of Glycyrrhiza uralensis. Results: Among the explants, the hypocotyl callus induction rate was the highest (94% on average), with the earliest occurrence and high degree of callus. The hypocotyls screened out were the best explants for callus induction. The callus differentiated to produce adventitious buds on MS + 6-BA (1.0-2.0) mg / L and NAA (0.5-1.0) mg / L medium; 2,4-D induced non-embryogenic callus, A certain concentration of 6-BA cooperated with NAA to induce embryogenic callus; light induced callus induction and growth. Conclusion: Different explants, hormone content and light are the main factors affecting the callus induction and growth of Glycyrrhiza uralensis.