Objective To investigate the relationship between Kashin Beck Disease and Human Parvovirus B 19 .Methods HPVB 19 DNA was detected in 55 sera of KBD patients and 52 healthy in adjacent non endemic area and 35 healthy sera in normal area using PCR and then linked the HPVB 19 DNA to pGEM T vector.The nucleotide sequence was analyzed and compared with HPVB 19 nucleotide sequence published by Genebank and another in Journal of virology.Results HPVB 19 DNA was found in 16 of 55 sera in KBD patients,and the HPVB 19 DNA position rate is significantly higher than that of the two healthy control groups.The nucleotide sequence homologies compared with the two published nucleotide sequence were 97.75%、97%,respectively.The putative amino acid homologies compared with the tow published were 93.5%.The amino acid variation was greater than the nucleotide sequence variation because of a base insertion.Conclusion There was a close relationship between HPVB 19 infection and Kashin Beck Disease. Objective To investigate the relationship between Kashin Beck Disease and Human Parvovirus B 19. Methods HPVB 19 DNA was detected in 55 sera of KBD patients and 52 healthy in adjacent non endemic area and 35 healthy sera in normal area using PCR and then linked the HPVB 19 DNA to pGEM T vector. The nucleotide sequence was analyzed and compared with HPVB 19 nucleotide sequence published by Genebank and another in Journal of virology. Results HPVB 19 DNA was found in 16 of 55 sera in KBD patients, and the HPVB 19 DNA position rate is significantly higher than that of the two healthy control groups. The nucleotide sequence homologies compared with the two published nucleotide sequences were 97.75%, 97%, respectively. The putative amino acid homologies compared with the tow published were 93.5%. The amino acid variation was greater than the nucleotide sequence variation because of a base insertion. Conlusion There was a close relationship between HPVB 19 i nfection and Kashin Beck Disease.