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目的初步探讨苹果多酚作微生态调节剂抑制变形链球菌,扶持血链球菌生长的实验研究,为龋病的防治提供一定的依据。方法采用抑菌环实验观察苹果多酚对变形链球菌和血链球菌生长的影响,检测其对变形链球菌产酸的变化,并通过扫描电镜观察对变形链球菌在玻片粘附情况的影响。结果苹果多酚能够明显抑制变形链球菌的生长,阻止其对玻片的粘附,降低其产酸能力,但对血链球菌生长没有明显的抑制作用。对变形链球菌与血链球菌的影响比较差异有统计学意义。结论苹果多酚具有调整窝沟致龋菌的作用,进一步研究很可能是一良好的窝沟菌群的微生态调节剂。
OBJECTIVE: To investigate the experimental study on apple polyphenols as a microecological regulator for the inhibition of Streptococcus mutans and the growth of Streptococcus sobrinus and provide a basis for the prevention and treatment of dental caries. Methods The effect of apple polyphenols on the growth of Streptococcus mutans and Streptococcus sanguis was observed with bacteriostasis test and the effect on the acid production of Streptococcus mutans was observed. The effect of Streptococcus mutans on glass slide adhesion was observed by scanning electron microscopy . Results apple polyphenols can significantly inhibit the growth of Streptococcus mutans to prevent its adhesion to glass slides and reduce its acid production capacity, but no significant inhibition of Streptococcus sobrinus growth. The impact of Streptococcus mutans and Streptococcus sanguisensis differences were statistically significant. Conclusion Apple polyphenols can regulate cariogenic bacteria in pit and furrow. Further study is likely to be a good micro-ecological regulator of pit and litter.