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目的:研究表达重组人肝增强团子(hALR)工程菌JM109的发酵工艺。方法:采用发酵罐发酵,优化工程菌发酵的培养基配方、pH值、诱导表达时间、补料方式等。结果:在pH6.9条件下,培养6h后诱导表达5h,同时补科,5L罐发酵工程菌,菌体收得量可达湿重33.0g/L。目标蛋白表达量约占菌体总蛋白的31.3%。结论:此发酵工艺可以较好地提高ALR工程菌菌体得率和目标蛋白表达量。
OBJECTIVE: To study the fermentation process of JM109 expressing recombinant human liver enhanced livers (hALR). Methods: fermenter fermentation was used to optimize the fermentation medium for engineering bacteria, pH value, induction time, feeding method and so on. Results: Under the condition of pH6.9, the cells were induced to express for 5h after culturing for 6h, while Bacillus subtilis and 5L cans fermented engineering bacteria, the yield of the cells reached 33.0g / L. The target protein expression accounted for about 31.3% of the total bacterial protein. Conclusion: This fermentation process can improve the yield of ALR engineered bacteria and the expression of target protein.