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目的合成玉米赤霉烯酮(ZEN)的全抗原。方法将烯醇化的ZEN与阳离子化的载体蛋白(BSA)偶联,制备高特异性的全抗原,用高性能基质辅助激光解吸电离-飞行时间质谱仪MALDI-TOF检测偶联物及载体蛋白的分子量,用商品化的ZEN免疫试剂盒对偶联物进行棋盘滴定验证。结果全抗原及载体蛋白BSA的分子量分别为69693.5 Da、66297.7 Da,平均每个BSA连上的ZEN分子个数为10.68个;平均每个OVA上连上的ZEN分子个数为5.32个;偶联物与免疫试剂盒中ZEN的抗体呈阳性反应。结论合成的ZEN全抗原为ZEN抗体的制备及免疫学方法的建立奠定了基础。
Objective To synthesize the full antigen of zearalenone (ZEN). Methods Enolase ZEN was coupled with cationized carrier protein (BSA) to prepare highly specific antigens. The conjugates and carrier proteins were detected by high performance matrix-assisted laser desorption / ionization-time of flight mass spectrometry (MALDI-TOF) The molecular weights were checked by checkerboard titration of conjugates using a commercial ZEN immunization kit. Results The molecular weight of whole antigen and carrier protein BSA were 69693.5 Da and 66297.7 Da, respectively. The average number of ZEN molecules attached to each BSA was 10.68. On average, the number of ZEN molecules attached to each OVA was 5.32. Antibodies to ZEN in the immunization kit were positive. Conclusion The synthetic ZEN antigen provides the basis for the preparation of ZEN antibody and the establishment of immunological methods.