作者利用质粒pGAL10-前S2S-1转化酵母CF52株,获得糖基化缺陷型、表达乙型肝炎病毒前S2和S抗原(前S2+S)的重组酵母株(mnn9株);质粒pYGAP前S2S-1转化酵母CF42株获得糖基化的、表达前S2+S的野型重组酵母株(MNN9株),后者可在前S2区的N4残基过度糖基化,于核心寡糖上附加大量甘露糖.以两种重组酵母进行发酵 The authors transformed the yeast CF52 strain with plasmid pGAL10-preS2S-1 to obtain a recombinant yeast strain (mnn9 strain) with glycosylation deficiency and expression of pre-S2 and S antigen of hepatitis B virus (preS2 + S); plasmid pYGAP preS2S -1 was transformed into yeast CF42 strain to obtain a glycosylated, wild-type, recombinant yeast strain (MNN9 strain) pre-S2 + S that was over-glycosylated at the N4 residue in the pre-S2 region and attached to the core oligosaccharide Large amounts of mannose Fermentation with two recombinant yeasts