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以中国科学院千烟洲生态站稻田红壤为研究对象,比较不同荧光校正方法对β-1,4-葡萄糖苷酶(βG)、β-1,4-N-乙酰葡糖氨糖苷酶(NAG)、酸性磷酸酶(AP)三种土壤酶活性结果影响。结果表明:(1)酸性土壤的βG、NAG、AP酶活性的测定结果为:曲线校正(CurQ)>单点校正(SinQ)>无土壤荧光淬灭校正(NQ)。(2)不同施肥种类的土壤,由三种校正方法所测得βG、NAG、AP的酶活性结果,其变化趋势一致,即βG、NAG酶活性均为施用猪粪(OM)土壤最高,施用氮磷钾化肥(NPK)土壤其次,不施任何肥料(CK)、秸秆还田(ST)土壤最低;AP活性则为NPK土壤明显低于CK、ST与OM土壤。(3)单点校正SinQ测得土壤荧光淬灭校正系数q值,与曲线校正CurQ所得值差异不显著,与曲线校正CurQ相比,单点校正SinQ法是一种准确、简便、低成本的酶活性测定方法,可以适用于大部分土壤,在具体应用时需要进一步试验验证。
In this paper, the red soil of paddy field in Qianyanzhou Ecological Station of Chinese Academy of Sciences was studied. The effects of different fluorescence correction methods on β-1,4-glucosidase (βG), β-1,4-N-acetylglucosaminidase , Acid phosphatase (AP) three kinds of soil enzyme activity results. The results showed that: (1) The results of determination of βG, NAG and AP enzyme activities in acidic soil were: CurQ> SinQ> NQ. (2) The results of enzyme activity of βG, NAG and AP measured by three calibration methods showed that the trend of change was the same, that is, the activities of βG and NAG were the highest in OM soil, NPK soil, followed by no fertilizer (CK), straw (ST) soil was the lowest, while AP activity was lower than that of CK, ST and OM soil. (3) Compared with curve-corrected CurQ, the single-point correction SinQ method is an accurate, simple and low-cost Determination of enzyme activity, can be applied to most of the soil, in the specific application requires further test validation.