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采用ＳＤ大鼠２０只，１５只为实验组，５只作为对照。用微量注射器将６－羟基多巴（６－ＯＨＤＡ）８ｕｇ／４ｕｌ分别注入大鼠右侧黑质致密部（ＳＮｃ）和中脑腹侧背盖区（ＶＴＡ），观察大鼠行为变化及黑质和纹状体形态学改变。结果：注药４周后，毁损组动物经阿朴吗啡诱发１４只出现旋转，超过７转／分为１２只（占８０％），经反复测试结果稳定；旋转鼠行Ｎｉｓｌ染色发现，右侧ＳＮｃ和ＶＴＡ区神经元数目明显减少；酪氨酸羟化酶（ＴＨ）免疫组织化学染色见：右侧ＳＮｃ及纹状体区ＴＨ免疫反应（ＴＨ－ＩＲ）数目较对侧明显下降。对照组则无旋转行为和形态学改变。提示这种改进的方法建立大鼠帕金森氏病模型成功率明显提高。
Twenty SD rats were used, 15 rats in the experimental group and 5 rats in the control group. (6-OHDA) 8ug / 4ul were injected into SNc and ventral tegmental area (VTA) of rat right ventricle respectively with microinjector to observe the change of behavior and substantia nigra And striatum morphological changes. Results: After 4 weeks of injection, 14 animals induced by apomorphine in the damaged group appeared rotation, more than 7 turns / divided into 12 (80%), the results of the test were stable after repeated injection. Nisl staining showed that the right side The numbers of neurons in SNc and VTA regions were significantly decreased. Immunohistochemical staining of tyrosine hydroxylase (TH) showed that the number of TH-IR in the right SNc and striatum was significantly decreased compared with the contralateral. The control group had no rotational behavior and morphological changes. Prompted that this improved method to establish rat Parkinson’s disease model significantly increased the success rate.