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测定并观察了49只高胆固醇食、高糖食和正常食对新西兰白兔脑缺血后血脂、脂质过氧化物(LPO)含量的影响,并提取血浆低密度脂蛋白(LDL)制成氧化型低密度脂蛋白(oxLDL),用薄层层析法分析了其脂质成分变化。结果示:胆固醇组血清游离脂肪酸(FFA)、朋固醇(CH)和磷脂(PL)含量显著增高,且脑缺血后增高更显著(P均<0.01),血浆和LDL的LPO含量亦显著增高。胆固醇食组TLC中X带(6.4/±2.6%)显著高于正常食组(2.8%±0.8%)和糖水组(1.2%±1.3%),P均<0.01。经Cu ̄(2+)溶液透析后,LFFA及PL带增高,而胆固醇酯(CE)及甘油三酯(TG)带减少。结果表明,高胆固醇食及脑缺血可导致脂质代谢紊乱,导致较多LPO和oxLDL生成,从而加速动脉粥样硬化和脑卒中。TLC中的X带可作为衡量脂质过氧化物的一个重要指标。
The effects of hypercholesterolemia, hyperglycemia and normal diet on blood lipids and lipid peroxides (LPO) in New Zealand white rabbits were measured and observed. Plasma low density lipoprotein (LDL) was extracted Oxidized low density lipoprotein (oxLDL) was assayed for lipid composition by thin layer chromatography. The results showed that the levels of serum free fatty acid (FFA), palmitol (CH) and phospholipid (PL) were significantly increased in cholesterol group and increased significantly after cerebral ischemia (all P <0.01). LPO content in plasma and LDL Also significantly higher. X-band (6.4 / ± 2.6%) in TLC group was significantly higher than that in normal diet group (2.8% ± 0.8%) and syrup group (1.2% ± 1.3% P <0.01. After dialysis with Cu 2+, the LFFA and PL bands increased, while the cholesteryl ester (CE) and triglyceride (TG) bands decreased. The results show that hypercholesterolemia and cerebral ischemia can lead to dyslipidemia, resulting in the production of more LPO and oxLDL, thereby accelerating atherosclerosis and stroke. X-band in TLC can be used as an important indicator of lipid peroxides.