羟基脲(HU)与氨基普鲁士蓝(TPF)-Na3[Fe(CN)5NH3]在一定条件下形成稳定的有色络合物,在500 nm处有强的吸收,适用于HU的测定。本工作用TPF作显色剂,研究了显色温度、缓冲溶液的用量、TPF用量等因素对比色法测定溶液中HU浓度的影响。 在5 mL容量瓶中加入1～2 mL pH≈5的磷酸缓 Hydroxylurea (HU) and aminofluorescein (TPF) -Na3 [Fe (CN) 5NH3] form stable colored complex under certain conditions and have strong absorption at 500 nm, which is suitable for the determination of HU. In this work, TPF was used as the color reagent to study the effect of color temperature, the amount of buffer solution, the amount of TPF and other factors on the determination of HU concentration in solution. In a 5 mL volumetric flask add 1 ~ 2 mL ph≈5 phosphate buffer