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目的研究肥胖导致铁营养不良的机制,为防治肥胖所致铁营养不良提供理论依据。方法选取雄性Wistar大鼠27只,随机分为对照组(n=10)和肥胖组(n=17),分别喂养普通饲料和高脂饲料,12 w后处死。取肝脏称重,液氮保存。测定肝脏铁蛋白、铁调素、转铁蛋白基因表达量。结果肥胖组大鼠肝脏中铁蛋白基因表达量(142.53±140.26)与对照组(1.00±0.00)比明显增高,铁调素基因表达量(0.36±0.31)与对照组(1.00±0.00)比明显降低,转铁蛋白基因表达量(3.65×10~5±2.53×10~5)与对照组(1.00±0.00)明显增加,差异均有统计学意义(均P<0.05)。结论肥胖大鼠肝脏与铁有关蛋白基因(例如铁蛋白、铁调素、转铁蛋白基因)的表达量发生改变,导致机体铁营养不良。
Objective To study the mechanism of obesity leading to iron malnutrition and provide a theoretical basis for prevention and treatment of iron malnutrition caused by obesity. Methods Twenty-seven male Wistar rats were randomly divided into control group (n = 10) and obesity group (n = 17), fed with normal diet and high-fat diet respectively and sacrificed 12 weeks later. Take the liver weighed, liquid nitrogen preservation. Liver ferritin, hepcidin, transferrin gene expression was measured. Results The gene expression of ferritin in obesity group was significantly higher than that in control group (142.53 ± 140.26 vs 1.00 ± 0.00), and the hepcidin gene expression level (0.36 ± 0.31) was significantly lower than that in control group (1.00 ± 0.00) (3.65 × 10 ~ 5 ± 2.53 × 10 ~ 5) and the control group (1.00 ± 0.00), the difference was statistically significant (all P <0.05). Conclusion The expression of iron-related protein genes (such as ferritin, hepcidin, and transferrin) in the liver of obese rats is changed, leading to iron malnutrition in the body.