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采用Plackett-Burman试验设计法及响应面分析法(RSM),对固定化Bacillus sp.ULi-11菌株生物不对称合成(R)-α-羟基苯乙酸((R)-HPA)进行优化.首先采用Plackett-Burman试验设计筛选影响产率的主要因素,再用最陡爬坡方法逼近最大响应区域后用Box-Behnken试验设计及RSM进行回归分析,得到的最佳条件为温度33℃,增殖时间9.3h,装液量18 mL,转速180 r/min,pH 7.2,接种量2%,种龄16 h,在此条件下(R)-HPA的产率为49.61%,比优化前的产率提高了23.7%.图3表6参16
The asymmetric synthesis of (R) -α-hydroxyphenylacetic acid ((R) -HPA) from immobilized Bacillus sp. ULI-11 strain was optimized by Plackett-Burman design and response surface methodology Plackett-Burman experimental design was used to screen the main factors influencing yield. After the steepest ascent method was used to approximate the maximum response area, the regression analysis was carried out with the Box-Behnken experimental design and RSM. The optimum conditions were as follows: temperature 33 ℃, The yield of (R) -HPA was 49.61% under the conditions of 9.3 h, 18 mL, 180 r / min, pH 7.2, inoculation 2% Increased by 23.7%