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研究了影响棉花钾吸收动力学参数的因素,初步确定了钾吸收动力学方法在棉花上应用的条件,比较了转基因抗虫棉新棉99B和常规棉中棉所35苗期的钾吸收特性。结果表明,棉花钾吸收动力学参数受到苗龄、耗竭液中起始钾浓度及培养液中钾浓度的显著影响,在苗龄较小(3～4叶苗与4～5叶苗相比)、耗竭液中起始钾浓度较高(0.35与0.2mmol·L1相比)、培养液中钾浓度较高(2.0与0.5mmol·L1相比)的情况下,Km、Cmin均比较高,而Imax在前两种条件下较高、在后一种条件下较低。在5～6叶期,新棉99B的Imax显著降低于中棉所35,而Km、Cmin显著高于后者(培养液中K+为0.5mmol·L1),耗竭液中起始K+为0.2mmol·L1),说明新棉99B吸收有限钾的能力低于中棉所35。
The factors influencing potassium absorption kinetics parameters of cotton were studied. The potassium absorption kinetics method was preliminarily applied to cotton, and the potassium absorption characteristics of transgenic cotton (New Cotton 99B) and conventional cotton (CCRI) 35 were compared. The results showed that the kinetic parameters of potassium uptake were significantly affected by the seedling age, the initial potassium concentration in the depletion solution and the potassium concentration in the culture solution. At the seedling age, the kinetic parameters of potassium uptake were smaller (3-4 leaflets compared with 4-5 leaflets) , The initial concentration of potassium in depleted liquid was higher (0.35 compared with 0.2 mmol·L1), Km and Cmin were higher in the culture medium with higher potassium concentration (2.0 vs. 0.5 mmol·L1) Imax is higher in the first two conditions and lower in the latter. At 5 ~ 6 leaf stage, the Imax of new cotton 99B was significantly lower than that of CIMC cotton, while the Km and Cmin were significantly higher than those of the latter (K + 0.5mmol·L1 in the culture medium). The initial K + was 0.2mmol · L1), indicating that the ability of New Cotton 99B to absorb limited potassium is lower than that of CIMC.