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目的：研究实验性视网膜光化学损伤中蛋白激酶Ｃ（ｐｒｏｔｅｉｎｋｉｎａｓｅＣ，ＰＫＣ）活性的改变；探索地塞米松（ｄｅｘａｍ－ｅｔｈａｓｏｎｅ，ＤＸＭ）对视网膜中ＰＫＣ活性的影响。方法：４８只ＳＤ大鼠分为对照组和ＤＸＭ组，后者于光照前３天开始，连续５天腹膜腔注射ＤＸＭ１ｍｇ／（ｋｇｄ）。经（１９００±１０６．９）ｌｘ的绿色荧光灯（λ＝５１０ｎｍ～５６０ｎｍ）连续照射２４小时后的６小时和１、３、７、１４天，进行视网膜ＰＫＣ活性检测。结果：光化学损伤后视网膜中的ＰＫＣ活性在短暂的上升后即出现持续性的下降；ＤＸＭ对ＰＫＣ的活性改变无明显作用。结论：光化学损伤中的视网膜功能障碍可能与ＰＫＣ活性的持续降低有关。
Objective: To study the changes of protein kinase C (PKC) activity in experimental retinal photochemical damage and to explore the effect of dexamethasone (DXM) on PKC activity in the retina. Methods: 48 SD rats were divided into control group and DXM group. The latter started 3 days before light irradiation and injected DXM1mg / (kgd) intraperitoneally for 5 consecutive days. The retinal PKC activity was measured 6 hours and 1, 3, 7 and 14 days after continuous irradiation with (1900 ± 106.9) lx green fluorescent lamps (λ = 510-560 nm). RESULTS: The PKC activity in the retina after photochemical injury showed a continuous decrease after a brief increase. DXM had no significant effect on the PKC activity. CONCLUSION: Retinal dysfunction in photochemical damage may be related to the continued decrease of PKC activity.