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长庆油田采用美国ＮＰＣ公司的商品微生物，分两次在３２口井上进行了１１６井次的微生物单井吞吐采油试验，效果显著。通过测定微生物处理前后油井产出的原油组成（含蜡量和烃组成）和物性（凝固点、粘度）、原油伴生水中有机酸含量和水的表面张力等的变化，以及在室内将油井产出的含水原油与微生物混合培养后测定生成气体的组成，研究了微生物对原油的作用：产生Ｃ６以下气态烃、少量ＣＯ２、Ｎ２和Ｈ２，使原油轻质化，产生有机酸和其他表面活性物质。本文报告了此项研究的结果。
Changqing Oilfield using the United States NPC’s commercial micro-organisms, in two wells in a total of 116 wells in 116 wells single-well huff and puff test, the effect is remarkable. Through the determination of crude oil composition (wax content and hydrocarbon composition) and physical properties (freezing point and viscosity) produced by oil well before and after microbial treatment, changes of organic acid content in crude associated water and surface tension of water, etc., The compositions of the produced gas were measured after mixed culture of crude oil and microorganism. The effects of microorganisms on crude oil were studied: gaseous hydrocarbons below C6, a few of CO2, N2 and H2 were produced, which made the crude oil lighter and produced organic acids and other surface active substances. This article reports the results of this study.