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用雄性化雌鱼(XX-?)的精于使卵子受精、2.5分钟后热休克处理受精卵的方法生产全雌三倍体.三倍体诱导的鉴定采用红血球核体积测量法.对照组二倍体的卵巢充满卵母细胞,而三倍体卵巢中仅含1～2个发育不良的卵母细胞,这表明雌性三倍体的不育性.正常三倍体化的主要缺点是会偶然产生一些二倍体(?+?),而全雌三倍体化则可避免与正常三倍体化有关的一些问题.
Whole-estrus triploids were produced by fertilization of ovum with fertilized female (XX-?) And heat-treated fertilized eggs 2.5 minutes later.Identification of triploid induction was made using the hematocrit volume measurement The ploidy of the ovary is full of oocytes, whereas the triploid ovary contains only 1 or 2 dysplastic oocytes, indicating female sterility. The major disadvantage of normal triploidization is that it can happen by chance Some diploid (? +?) Is produced, while all-female triploidization avoids some of the problems associated with normal triploidization.