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目的 探讨内毒素诱导的肺损伤与Th1/Th2平衡的关系。方法 采用颈静脉注入 ,LPS复制大鼠急性肺损伤模型 ,用ELISA法检测其外周血单个核细胞 (PBMC)产生IFN γ、IL 4水平及IFN γ/IL 4比值。结果 与正常对照组相比 ,急性肺损伤组外周血PBMC产生IFN γ水平和IFN γ/IL 4比值明显升高 (P<0 0 5 ;P <0 0 0 1) ,而IL 4水平差异无显著性 (P >0 0 5 ) ,肺组织病理切片显示大鼠发生ALI。结论 LPS诱导的肺损伤 ,体内存在Th1/Th2类细胞因子水平失衡
Objective To investigate the relationship between endotoxin-induced lung injury and Th1 / Th2 balance. Methods The acute lung injury model was induced by injection of the jugular vein and LPS. The levels of IFN - γ and IL - 4 and the ratio of IFN γ / IL 4 in peripheral blood mononuclear cells (PBMCs) were measured by ELISA. Results Compared with the normal control group, the level of IFN-γ and the ratio of IFN-γ / IL-4 in PBMC of acute lung injury group were significantly increased (P <0.05 or P <0.01), while the level of IL-4 was no difference Significant (P> 0.05), lung tissue pathology showed ALI in rats. Conclusion LPS-induced lung injury has imbalance of Th1 / Th2 cytokine levels in vivo