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为探讨使用木麻黄-内生真菌共生体应对连栽障碍的可能性,在前期已获得2种木麻黄化感物质(槲皮黄素-3-α-阿拉伯糖苷,Q3A;槲皮黄素-3-β-葡萄糖苷,Q3B)胁迫对木麻黄(Endophyte-free,EF)幼苗小枝活性氧代谢和清除系统影响的基础上,本研究以感染(Endophyte-infected,EI)内生真菌Aspergillus sp.的木麻黄水培幼苗(Casuarina equisetifolia Forst.)为实验材料,在控制环境水培条件下,分析内生真菌对Q3A和Q3B胁迫下木麻黄幼苗小枝活性氧代谢和清除系统的影响.结果显示,内生真菌的侵染能够有限缓解木麻黄化感物质的胁迫,在中度轻度及短期胁迫(100 mg/L 0-12 h、50 mg/L 0-24 h、25 mg/L 0-36 h、12.5 mg/L 0-48 h)下有明显的效果,表现在与木麻黄EF相比,木麻黄EI活性氧含量显著下降,丙二醛(MDA)含量显著降低,保护酶活性显著增加;内生真菌缓解化感物质胁迫的有效浓度和胁迫期与木麻黄EF能够耐受的化感物质胁迫浓度和胁迫期一致;Q3A胁迫下木麻黄EI保护酶活性显著高于Q3B胁迫下的提升幅度.说明内生真菌提升了宿主木麻黄的抗逆性,对宿主应对Q3A胁迫的贡献更大,推测内生真菌对木麻黄EI保护酶系统的提升需要建立在木麻黄保护酶系统合成和降解还未受到不可逆伤害的前提下.(图6表1参40)
In order to explore the possibility of using Casuarina equisetifolia-endophytic fungi symbionts to deal with the continuous planting obstacles, two kinds of allelopressin (quercetin -3-α-arabinoside, Q3A, quercetin-3- β-glucoside, Q3B) on the active oxygen metabolism and scavenging activity of branchlets of Endophyte-free (EF) seedlings, we used the wood of Endophyte-infected (EI) endophyte Aspergillus sp. Casuarina equisetifolia Forst. The effects of endophytic fungi on the active oxygen metabolism and scavenging system of spruce of Casuarina equisetifolia seedlings under the stress of Q3A and Q3B were analyzed under the condition of controlled environment hydroponics. The results showed that endogenous Infection with fungi could relieve the stress caused by allelochemicals of Casuarina equisetifolia, moderately mild and short-term stress (0 mg / L 0-12 h, 50 mg / L 0-24 h, 25 mg / L 0-36 h , 12.5 mg / L 0-48 h). Compared with Casuarina equisetifolia, the content of reactive oxygen species (EI) and the content of malondialdehyde (MDA) in Casuarina equisetifolia decreased significantly and the activity of protective enzyme increased significantly. Effective concentrations of endophytic fungi alleviating allelochemicals and allergens that are tolerant to Casuarina equisetifolia during stress periods The stress concentration and stress period were the same; Q3A stress under the protection of the activity of EI protective enzyme was significantly higher than the Q3B stress enhance the extent. Endophytic fungi enhance the virulence of the host Casuarina, the host contributes to Q3A stress more, Presumably endophytic fungi on the Casuarina EI protective enzyme system needs to be established on the premise of the Casuarina equisetifolia protective enzyme system synthesis and degradation has not yet been irreversibly harmed (Figure 6 Table 1 Reference 40)