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目的通过对180株金黄色葡萄球菌临床分离株MecA、Nuc基因的检测,以期选择出一种适合临床的并能简便、快速、准确检测耐甲氧西林金黄色葡萄球菌(methicillin resistant Staphylococcus aureus,MRSA)的方法。方法细菌鉴定及药敏采用全自动细菌鉴定和药敏分析仪。MecA、Nuc基因检测采用荧光定量聚合酶链反应(fluorescence quantitative polymerase chain reaction,FQ-PCR)法。结果 FQ-PCR对180株金黄色葡萄球菌MecA、Nuc基因检出率为75.0%,全自动细菌鉴定和药敏分析仪对180株金黄色葡萄球菌的MRSA检出率为72.8%,2种检测方法比较差异无统计学意义(P>0.05)。全自动细菌鉴定和药敏分析仪对MRSA的检测正确率为94.8%。结论 FQ-PCR检测MRSA正确率高,只需要1~2 h即可完成。该方法快速、简便、准确,值得推广应用。
OBJECTIVE To detect the MecA and Nuc genes of 180 Staphylococcus aureus clinical isolates in order to select a suitable clinical and simple, rapid and accurate detection of methicillin-resistant Staphylococcus aureus (MRSA )Methods. Methods Bacterial identification and drug sensitivity using automatic bacterial identification and drug sensitivity analyzer. MecA, Nuc gene detection using fluorescence quantitative polymerase chain reaction (FQ-PCR) method. Results The positive rate of MecA and Nuc gene in 180 strains of Staphylococcus aureus was 75.0% by FQ-PCR. The detection rate of MRSA for 180 strains of Staphylococcus aureus was 72.8% by automatic bacterial identification and susceptibility analyzer. There was no significant difference in the methods (P> 0.05). The detection rate of MRSA was 94.8% with automatic bacterial identification and drug susceptibility analyzer. Conclusion The accuracy of FQ-PCR in detecting MRSA is high, only need 1 ~ 2 h to complete. The method is rapid, simple and accurate, which is worth popularizing and applying.