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目的:探讨NOB1(nin one binding)在胰腺癌组织中的表达情况及与胰腺癌临床病理特征的关系。方法:①SYBR Green实时定量PCR和Western印迹法检测20例胰腺癌及癌旁对照、14例正常胰腺冰冻组织中NOB1基因mRNA及其编码蛋白的表达情况。②免疫组化回顾性检测50例胰腺癌石蜡切片中NOB1蛋白表达情况,并分析其与病人年龄、性别、肿瘤部位、病理分期、淋巴结转移等临床病理特征的相关性。结果:①20例胰腺癌中NOB1基因mRNA表达明显高于癌旁组织,P<0.05;16例(80%)胰腺癌组织中NOB1蛋白明显高于癌旁组织;而14例正常组织中NOB1蛋白阳性率仅14.3%。②免疫组化有34例呈(+++),8例呈(++),4例呈(+),4例呈(-),阳性表达率为84%,且NOB1在多数胰腺癌早期阶段即有强阳性表达。NOB1与肿瘤大小呈正相关(P<0.05)。NOB1与年龄、性别及淋巴结浸润无相关性(P>0.05)。结论:NOB1在胰腺癌组织中高表达,且在较早期胰腺癌中即有强阳性表达,提示可能与胰腺癌发生有密切关系。
Objective: To investigate the expression of NOIN (nin one binding) in pancreatic cancer and its relationship with clinicopathological features. Methods: ①SYBR Green real-time quantitative PCR and Western blotting were used to detect the expression of NOB1 mRNA and protein in 20 cases of pancreatic cancer and adjacent non-cancerous tissues and 14 cases of normal pancreatic frozen tissue. ② The immunohistochemistry was used to detect the expression of NOB1 protein in 50 cases of pancreatic cancer paraffin sections and to analyze the correlation with the clinicopathological features such as age, sex, tumor location, pathological stage and lymph node metastasis. Results: ① The mRNA expression of NOB1 in 20 cases of pancreatic cancer was significantly higher than that in adjacent non-cancerous tissues (P <0.05). The NOB1 protein in 16 cases (80%) of pancreatic cancer tissues was significantly higher than that in paracancerous tissues. NOB1 protein was positive in 14 cases The rate is only 14.3%. ② There were 34 cases (+++) in immunohistochemistry, (++) in 8 cases, (+) in 4 cases and (-) in 4 cases. The positive rate of NOB1 was 84% in most pancreatic cancer The stage is strongly positive. NOB1 was positively correlated with tumor size (P <0.05). There was no correlation between NOB1 and age, gender and lymph node infiltration (P> 0.05). CONCLUSION: NOB1 is overexpressed in pancreatic cancer tissues and strongly positive in early stage pancreatic cancer, suggesting that it may be closely related to the occurrence of pancreatic cancer.