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目的:探讨白细胞介素-1受体拮抗剂(interleukin-1 receptor antagonist,IL-1ra)基因型与冠心病(coronary heart disease,CHD)患者患慢性牙周炎(chronic periodontitis,CP)易感性的关系。方法:选取冠心病患者86例,其中冠心病伴慢性牙周炎患者45例,单纯冠心病患者41例。取颊黏膜拭子,提取DNA,采用序列特异引物聚合酶链反应(sequence specific primers-polymerase chain reaction,SSP-PCR)法测定IL-1RN内含子2/VNTR位点的基因型,比较各基因型检出率的差别,采用SPSS13.0软件包进行统计学处理。结果:IL-1RN内含子2/VNTR等位基因2在冠心病伴慢性牙周炎组的检出率显著高于单纯冠心病组,差异有统计学意义(χ2=4.960,P=0.026)。结论:在冠心病患者中,IL-1RN内含子2/VNTR等位基因2可能与其慢性牙周炎的遗传易感性相关。
Objective: To investigate the susceptibility to chronic periodontitis (CP) in patients with interleukin-1 receptor antagonist (IL-1ra) genotype and coronary heart disease (CHD) relationship. Methods: 86 patients with coronary heart disease were selected, including 45 cases of coronary heart disease with chronic periodontitis and 41 cases of simple coronary heart disease. The buccal mucosal swabs were extracted and the DNA was extracted. The genotype of IL-1RN intron 2 / VNTR locus was determined by sequence specific primer-polymerase chain reaction (SSP-PCR) Type detection rate differences, using SPSS13.0 software package for statistical analysis. Results: The detection rate of IL-1RN intron 2 / VNTR allele 2 in CHD patients with chronic periodontitis was significantly higher than that in CHD patients (χ2 = 4.960, P = 0.026) . CONCLUSIONS: IL-1RN intron 2 / VNTR allele 2 may be associated with the genetic predisposition to chronic periodontitis in CHD patients.