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目的:了解小鼠生后不同时相海马组织中TFF3的表达规律。方法:本研究以生后P0、P1、P3、P5、P7、P9、P11、P13、P15、P17、P19、P21天昆明种小鼠为实验对象,参照王平宇的脑读片图片取大脑4-6段,进行石蜡包埋、切片厚6μm、HE染色和TFF3免疫组织化学染色,小鼠海马组织结构及该段核团进行定位及该段脑组织中TFF3的分布及变化。另取不同时相小鼠海马组织液氮冻存,提取组织总RNA,反转录,RT-PCR检测TFF3mRNA的转录水平。结果:1.TFF3在海马CA1、CA2、CA3、CA4及齿状回神经细胞的细胞质与细胞核中均有表达,随时间推移,细胞质阳性信号增强,P15开始细胞核呈阴性表达,且CA1~CA4区阳性神经元减少;第三脑室和侧脑室脉络丛上皮、丘脑内侧背核、外侧背核、缰内侧核、外侧膝状体背核、丘脑后核、丘脑腹后内侧核、外侧核、中央内侧核等也呈TFF3免疫反应阳性,且表达趋势和海马相似;内囊有大量TFF3阳性纤维存在。2.随着个体的发育,P15后海马组织中TFF3mRNA的表达量逐渐减少,与P1~P13相比P<0.01。结论:TFF3参与小鼠海马和丘脑各核团的发育,表达模式随出生时间推移TFF3表达逐渐减少。
Objective: To investigate the expression of TFF3 in hippocampus of mice at different time points after birth. Methods: Kunming mice of P0, P1, P3, P5, P7, P9, P11, P13, P15, P17, P19 and P21 after birth were enrolled in this study. 6 sections were paraffin embedded, section thickness 6μm, HE staining and TFF3 immunohistochemical staining, the structure of the hippocampus of mice and the nucleus were located and the distribution and changes of TFF3 in this section of brain tissue. In addition, the different time phase of mouse hippocampal tissue was stored in liquid nitrogen, total RNA was extracted, reverse transcription, RT-PCR detection of TFF3mRNA transcription. TFF3 was expressed in the cytoplasm and nucleus of hippocampal CA1, CA2, CA3, CA4 and dentate gyrus neurons. Over time, cytoplasm positive signal was enhanced, P15 nucleus was negative, and CA1 ~ CA4 Positive neurons; third ventricle and lateral ventril choroid plexus, medial dorsal thalamic nucleus, lateral dorsal nucleus, medial hallux nucleus, lateral geniculate nucleus dorsal nucleus, posterior thalamic nucleus, medial thalamic nucleus, lateral nucleus, central medial Nuclear TFF3 immunoreactivity was also positive, and the expression trend and the hippocampus similar; a large number of TFF3-positive cysts within the capsule. With the development of individuals, the expression of TFF3mRNA in P15 hippocampus gradually decreased, P <0.01 compared with P1 ~ P13. CONCLUSION: TFF3 is involved in the development of various nuclei in the hippocampus and thalamus in mice. The expression of TFF3 gradually decreases with the passage of time.