论文部分内容阅读
目的:研究Smad 4 mRNA在去卵巢大鼠下丘脑、垂体中的表达水平和意义。方法:SD大鼠分为3组(每组10只):对照组(月龄<24周雌性大鼠)、去势组(手术切除双侧卵巢大鼠)、治疗组(去势后雌激素治疗雌性大鼠30天),动物处死后迅速取材,提取各组大鼠下丘脑、垂体组织RNA,反转录成cD NA,下丘脑,采用实时定量PCR方法(Real-time quantitative reverse transcriP tion Polymerase chain reaction;qRT-PCR),检测各组大鼠Smad4 mRNA在下丘脑及垂体中的表达。结果:去势组大鼠下丘脑Smad4 mRNA水平是正常组0.4±0.22倍(P<0.05),雌激素治疗组大鼠下丘脑Smad4 mRNA水平是正常组2.5±0.79倍(P<0.05);去势组大鼠垂体Smad4 mRNA水平是正常组0.2±0.12倍(P<0.05),垂体中Smad4 mRNA水平是正常组8.8±0.16倍(P<0.05)。结论:去卵巢大鼠下丘脑、垂体表达Smad 4基因,并与雌激素水平变化相关。
Objective: To investigate the expression and significance of Smad 4 mRNA in the hypothalamus and pituitary of ovariectomized rats. Methods: The SD rats were divided into 3 groups (10 in each group): control group (24-week-old female rats), castration group (bilateral ovariectomized rats), treatment group The female rats were treated for 30 days. After the animals were sacrificed, the animals were quickly taken and the RNA of hypothalamus and pituitary tissue was extracted from each group. The RNA was reverse transcribed into cD NA and hypothalamus. Real-time quantitative reverse transcript Polymerase qRT-PCR) was used to detect the expression of Smad4 mRNA in hypothalamus and pituitary. Results: The level of Smad4 mRNA in the hypothalamus of the castration group was 0.4 ± 0.22 times of the normal group (P <0.05), and the level of Smad4 mRNA in the hypothalamus of the estrogen-treated group was 2.5 ± 0.79 times of the normal group (P <0.05) The potential level of Smad4 mRNA in pituitary was 0.2 ± 0.12 times that of normal group (P <0.05), and the level of Smad4 mRNA in pituitary was 8.8 ± 0.16 (P <0.05). Conclusion: The expression of Smad 4 gene in the hypothalamus and pituitary of ovariectomized rats is related to the estrogen level.