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介绍一种用合成的多抗原肽免疫动物来制备与天然人心肌肌钙蛋白Ⅰ特异反应的单克隆抗体新方法。从人心肌肌钙蛋白ⅠＮ端，根据多肽分子氨基酸的亲水性值选出了ＰＡＰＡＰＩＡＡＡＳＳ１１个氨基酸片段。将它用有八位点的赖氨酸核连接成多抗原肽后，以它为抗原用Ｒｉｂｉ佐剂进行免疫。将小鼠脾细胞与ＳＰ２／０骨髓瘤细胞融合，获１１株抗此多肽的杂交瘤细胞株。对其中７株属于ＩｇＧ亚类的细胞进行了ＥＬＩＳＡ分析，发现这７株细胞均与天然的人心肌肌钙蛋白Ⅰ呈特异性反应，而不与人骨骼肌肌钙蛋白Ⅰ发生交叉反应。利用３Ａ２细胞株所分泌的单克隆抗体对急性心肌梗塞患者、手术患者和正常人的血样进行了ＥＬＩＳＡ检测，证明它只对心肌梗塞患者血清发生阳性反应。获得了较为满意的结果。此细胞株所分泌的抗体有望用于心肌梗塞临床检测。
A new method for the preparation of monoclonal antibodies that specifically react with native human cardiac troponin I by immunizing animals with a synthetic polyepitope peptide is described. From human cardiac troponin I N-terminal, PAPAPIAAASS11 amino acid fragments were selected based on the amino acid hydrophilicity of the polypeptide molecule. After it was ligated to a multi-antigen peptide with an eight-site lysine nucleus, it was immunized with the Ribi adjuvant as an antigen. The mouse spleen cells were fused with SP2 / 0 myeloma cells, and 11 hybridoma cell lines against this polypeptide were obtained. Seven of the cells belonging to the IgG subclass were subjected to ELISA analysis and found that all of the seven cells specifically reacted with native human cardiac troponin I without cross-reacting with human skeletal troponin I. The monoclonal antibody secreted by 3A2 cell line was used to detect the blood samples of acute myocardial infarction patients, surgical patients and normal persons by ELISA, which proved that it only had a positive reaction to the serum of myocardial infarction patients. Obtained a more satisfactory result. Antibodies secreted by this cell line are expected to be used in clinical testing of myocardial infarction.