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哺乳动物体内含有5型常见的乳酸脱氢酶(LDH)同功酶,是由亚基A和B组成的纯四聚体或杂化四聚体,即:LDH_1(B_4)、LDH_2(B_3A)、LDH_3(B_2A_2)、LDH_4(BA_3)和LDH_5(A_4)。1963年Blanco和Goldberg等发现在多种哺乳动物和鸟类睾丸组织及精子中还含有另一种LDH-同功酶,称为LDH_x(C_4)。电泳分析表明,LDH_x的分布有明显的种族特异性:人、小白鼠、兔和狗的LDH_x活性区带为单一区带,且人的LDH_x活性区带位于LDH_3与LDH_4之间;大白鼠、豚鼠和牛的多条区带;鸡、鸭、羊、猫、猪则无此特殊的电泳区带。据文献记载,运用电泳方法,可检测出精
Mammals contain type 5 common lactate dehydrogenase (LDH) isozymes and are pure tetramers or hybrid tetramers consisting of subunits A and B, namely LDH_1 (B_4), LDH_2 (B_3A) , LDH_3 (B_2A_2), LDH_4 (BA_3) and LDH_5 (A_4). In 1963 Blanco and Goldberg et al. Found that LDH_x (C_4) was also found in many mammalian and bird testis tissues and sperm. The results of electrophoresis showed that the distribution of LDH_x was significantly racially specific: the LDH_x activity band of human, mouse, rabbit and dog was single band, and the LDH_x activity band of human was located between LDH_3 and LDH_4; And cattle multiple zones; chicken, duck, sheep, cats, pigs do not have this special electrophoresis zone. According to the literature, the use of electrophoresis, can detect fine