目的探讨儿童全血常量元素(钙、镁)和微量元素(铁、锌、铜)含量的年龄差异,并建立临床正常参考范围。方法以20946例健康查体的儿童(男12447例,女8499例)为对象,按照年龄分为四组(﹤1岁,1岁～,3岁～,﹥6岁)。采用原子吸收光谱法测定末梢血钙、镁、铁、锌、铜元素的含量。计算出各年龄组、各元素含量第3、10、25、50、75、90、97百分位的相应数值。结果不同年龄组之间血钙、镁、铁、锌、铜的含量有显著性差异(P﹤0.05),且随年龄的变化而变化。便于临床应用,取第25～75百分位的相应数值作为正常参考范围。结论判断5种元素含量是否正常应考虑年龄因素,各年龄组需分别参考其相应的正常范围。 Objective To investigate the age differences of children’s total blood elements (calcium, magnesium) and trace elements (iron, zinc, copper) content and to establish the clinical normal reference range. Methods Totally 20946 healthy children (12447 males and 8499 females) were divided into four groups according to their age (<1 year old, 1 year old, 3 years old,> 6 years old). The contents of calcium, magnesium, iron, zinc and copper in peripheral blood were determined by atomic absorption spectrometry. The corresponding values ​​of the 3rd, 10th, 25th, 50th, 75th, 90th and 97th percentiles of each age group were calculated. Results The levels of serum calcium, magnesium, iron, zinc and copper in different age groups were significantly different (P <0.05), and changed with age. For clinical applications, take the 25th to 75th percentile values ​​as the normal reference range. Conclusion To determine whether the five elements are normal age should be considered, all age groups need to refer to the corresponding normal range.