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以小叶龙竹种子为外植体,通过研究MS培养基中不同植物生长调节剂浓度组合对外植体愈伤组织诱导和不定芽分化的影响以及不同配比对生根的作用,建立了稳定的繁殖再生体系。试验结果表明愈伤组织诱导的最适培养基为MS+2,4-D5·0mg·L-1;不定芽分化的最适培养基为MS+2,4-D0·5mg·L-1+6-BA1·0mg·L-1+KT0·25mg·L-1;小苗的最适生根培养基为1/2MS+6-BA0·5mg·L-1+NAA1·0mg·L-1+IBA0·4mg·L-1。建立的繁殖再生体系将为进一步利用分子生物学技术对竹类植物进行遗传改良奠定基础。
In order to study the effects of different combinations of plant growth regulators (MSCs) on callus induction and adventitious bud differentiation of explants and the effects of different proportions on rooting, we established a stable breeding Regeneration system. The results showed that the optimum culture medium for callus induction was MS + 2,4-D5 · 0 mg · L-1. The optimum medium for adventitious bud differentiation was MS + 2,4-D0 · 5 mg · L-1 + 6-BA1 · 0 mg · L-1 + KT0 · 25 mg · L-1. The optimal medium for rooting was 1 / 2MS + 6-BA0 · 5 mg · L-1 + NAA1 · 0 mg · L-1 + IBA0 · 4mg · L-1. The establishment of breeding and regeneration system will lay the foundation for the further use of molecular biology techniques for genetic improvement of bamboo plants.