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Angucyclinones是一类主要由陆地放线菌产生的芳香聚酮类化合物,其生物合成主要是由Ⅱ型聚酮合酶(PKS)催化完成。为了在海洋放线菌中探索此类芳香聚酮类化合物,我们以167株海洋放线菌基因组DNA为模板,对含有Ⅱ型聚酮合酶的菌株进行了PCR筛选,通过对PCR产物测序验证,确定了12株阳性菌株。通过大规模发酵培养和色谱分离、波谱鉴定,从阳性菌株Streptomyces sp.PKU-MA00218中发现了四个angucyclinone芳香聚酮类化合物,6-deoxy-8-O-methylrabelomycin(1),8-O-methylrabelomycin(2),8-O-methyltetrangulol(3),和angucyclinone C的C环裂解产物(4)。化合物4为首次从海洋放线菌中分离得到。Angucyclinone芳香聚酮1–4的发现展示了基于生物合成的基因组挖掘技术在天然产物探索发现中的重要作用。
Angucyclinones are a class of aromatic polyketides mainly produced by terrestrial actinomycetes, whose biosynthesis is mainly catalyzed by type II polyketide synthase (PKS). In order to explore these aromatic polyketides in marine actinomycetes, 167 strains of Actinomycete marine actinomycete genomic DNA were used as templates for PCR screening of strains containing type II polyketide synthase. By sequencing the PCR products , Identified 12 positive strains. Four angucyclinone aromatic polyketides, 6-deoxy-8-O-methylrabelomycin (1) and 8-O-methylrabelomycin were found from the positive strain Streptomyces sp.PKU-MA00218 through large- methylrabelomycin (2), 8-O-methyltetrangulol (3), and C-ring cleavage products of angucyclinone C (4). Compound 4 was isolated from marine actinomycetes for the first time. The discovery of Angucyclinone aroma polyketides 1-4 demonstrates the important role of biosynthetic genome mining techniques in discovering natural products.