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目的探讨受体耐受性树突状细胞(DC)在诱导供体特异性免疫耐受中的作用及其可能机制。方法通过以B7反义肽封闭的负载供体抗原的受体DC对受体小鼠进行预处理,3d或2个月取脾脏分离T细胞,与负载供体抗原或无关供体抗原的DC作混合淋巴细胞反应,并且在3d或2个月时以负载供体抗原的受体DC对受体小鼠进行再次免疫后,RT-PCR半定量法测定脾脏中细胞因子mRNA表达。结果以B7反义肽封闭的负载供体抗原的受体DC预处理3d或2个月后的受体T细胞,产生对间接途径提呈的供体抗原的免疫低反应;同时脾脏IL-10表达升高,而IL-2、IFN-γ表达降低。结论 B7反义肽封闭的受体DC可能通过免疫偏移诱导针对间接途径提呈的供体抗原特异性的免疫低反应。
Objective To investigate the role of receptor-tolerant dendritic cells (DCs) in inducing donor-specific immune tolerance and its possible mechanism. Methods The recipient mice were pretreated with DCs loaded with donor antigen blocked with B7 antisense peptide. T cells were harvested from the spleens after 3 or 2 months, and incubated with DCs carrying donor or non-donor antigens The lymphocyte reaction was mixed and the recipient mice were re-immunized with the donor DC-loaded receptor DC at 3d or 2 months. The cytokine mRNA expression in the spleen was determined by RT-PCR semi-quantitative analysis. Results Donor T cells loaded with donor antigen blocked with B7 antisense peptide were pretreated with recipient T cells three or two months later to produce immunological hyporesponsiveness to indirectly presented donor antigens; meanwhile, spleen IL-10 While the expression of IL-2 and IFN-γ decreased. Conclusion B7 antisense peptide-blocked receptor DCs may induce immunological hyporesponsiveness to donor antigen presented by indirect pathway by immune migration.