日本血吸虫虫卵是主要的致病因子 ,但虫卵抗原的制备比较繁琐 ,需要大量的实验动物。为了探索和发展可用于诊断和疫苗的模拟虫卵抗原 ,我们采用噬菌体展示随机 15 肽文库对日本血吸虫虫卵单克隆抗体 6Ｂ12所识别的抗原表位进行了模拟。用 6Ｂ12作为“诱饵” ,采用生物淘金法通 Schistosoma japonicum eggs are the main virulence factors, but the production of egg antigen is complicated and requires a large amount of experimental animals. To explore and develop mimic antigens for diagnosis and vaccines, we used a phage-displayed random 15-peptide library to mimic the epitope recognized by Schistosoma japonicum egg monoclonal antibody 6B12. With 6B12 as a “decoy”, using biological gold rush method