天花粉蛋白（Ｔｒｉｃｈｏｓａｎｔｈｉｎ．以下简称ＴＣＳ）具有广谱抗病毒作用。利用ＣａＭＶ３５Ｓ启动子，ＧＵＳ报告基因，ＮＰＴ（筛选标记基因构建了植物表达载体ｐＢＩＴＧ２４。通过根癌农杆菌转化系统，将ＴＣＳ基因－ＧＵＳ融合基因导入烟草获得了转基因植株。采用Ｘ－Ｇｌｕｃ染色检测到ＧＵＳ基因在转基因烟草愈伤组织、植株的根、茎中都得到了表达。提取阳性烟草的总ＤＮＡ作模板，进行ＰＣＲ特异片段扩增，进一步证明ＴＣＳ基因整合入烟草基因组。 Trichosanthin (hereinafter referred to as TCS) has a broad spectrum of antiviral effects. The plant expression vector pBITG24 was constructed by using CaMV35S promoter, GUS reporter gene and NPT (screening marker gene), and the TCS gene-GUS fusion gene was introduced into tobacco through Agrobacterium tumefaciens transformation system, and transgenic plants were obtained by X-Gluc staining GUS gene was expressed in the transgenic tobacco callus, plant roots and stems, and the total DNA of positive tobacco was extracted as a template for PCR specific fragment amplification, further demonstrating that the TCS gene is integrated into the tobacco genome.