目的　克隆出血性大肠杆菌O15 7:H7的VT2毒素基因。方法　利用PCR技术从出血性大肠杆菌O15 7:H7染色体基因组中扩增出VT2毒素基因 ,并连接至pGEM -T载体上 ,构建重组质粒pGVT2并进行序列测定。结果与结论　酶切分析表明重组质粒含有VT2毒素基因 ,核苷酸序列分析表明 ,其序列与GenBank上O15 7:H7的VT2毒素基因一致 Objective To clone the VT2 toxin gene of hemorrhagic Escherichia coli O15 7: H7. Methods The VT2 toxin gene was amplified from the genome of hemorrhagic Escherichia coli O15 7: H7 by PCR and ligated into pGEM-T vector to construct the recombinant plasmid pGVT2. Results and Conclusion Enzyme digestion analysis showed that the recombinant plasmid contained the VT2 toxin gene. The nucleotide sequence analysis showed that the sequence of the VT2 toxin gene was consistent with the VT2 toxin gene of O15 7: H7 in GenBank